Protein_Domain
SspDnaB(C)
Part:BBa_K1362409:Design
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-06)
SspDnaB C-Intein cloning piece
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Part sequence and DNA were obtained from pCL20 by Prof. Henning D. Mootz, University of Muenster. [1]. Further Information came amongst other literature from Liu et al. [2]
References
[1] Mootz, H. D. Split inteins as versatile tools for protein semisynthesis. Chembiochem 10, 2579–89 (2009).
[2] Volkmann, G., Volkmann, V. & Liu, X.-Q. Site-specific protein cleavage in vivo by an intein-derived protease. FEBS Lett. 586, 79–84 (2012).