Protein_Domain
SspDnaB(C)

Part:BBa_K1362409:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-06)


SspDnaB C-Intein cloning piece


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.


Source

Part sequence and DNA were obtained from pCL20 by Prof. Henning D. Mootz, University of Muenster. [1]. Further Information came amongst other literature from Liu et al. [2]

References

[1] Mootz, H. D. Split inteins as versatile tools for protein semisynthesis. Chembiochem 10, 2579–89 (2009).

[2] Volkmann, G., Volkmann, V. & Liu, X.-Q. Site-specific protein cleavage in vivo by an intein-derived protease. FEBS Lett. 586, 79–84 (2012).